Present study was aimed to evaluate the phytochemical and anti-cancer activity of Nerium oleander leaves in selected cell lines in-vitro. Nerium oleander leaves were collected from local cultivating areas near to Anantapuramu, shade dried, mechanically made into powder. The powder was subjected to extraction by cold maceration method. The methanolic extract of Nerium oleander leaves was subjected to preliminary pharmacognostic and phytochemical screening using standard methods. In-vitro anticancer activity of Nerium oleander leaves was performed on K-562-Human chronic myelogenous leukaemia, Bone marrow, A549 - Human lung carcinoma and LNCap-FGC - Human Carcinoma Prostate by MTT assay method at a concentration of 50, 100 and 200 micrograms per ml using MTT assay. The percentage yield of Nerium oleander leaves found to be 18 % w/w. Preliminary phytochemical screening of Nerium oleander leaves revealed the presence of tannins, alkaloids, glycosides and flavonoids. In anti-cancer activity, the plant extract showed IC50 value of 169.06 µgm/ml for K-562-Human chronic myelogenous leukaemia, Bone marrow, 156.15 µgm/ml for A549 - Human lung carcinoma and 145.85 µgm/ml for LNCap-FGC - Human Carcinoma Prostate. The present study reveals the anti-cancer activity of Nerium oleander leaves.
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